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KMID : 0357919690030010009
Korean Journal of Pathology
1969 Volume.3 No. 1 p.9 ~ p.12
The Determination of Blood Sugar by Glucose Oxidate Method
±èÀç½Ä(ÐÝî¤ãÕ)/Jae Sik Kim
À±¼ºÁ÷(ëÅá¡òÃ)/¹ÚÈñ¼÷(ÚÓý÷â×)/¹ÚÁؽÄ(ÚÓñÕãÕ)/¿Àº¹µæ(çïÜØÔð)/Seong Jik Yoon/Hi Sook Park./Joon Shik Park/Bok Duk Oh
Abstract
Since high degree of specificity of glucose oxidase for beta-glucose had been
demonstrated, the use of the enzyme, glucose oxidase and peroxidase coupled with a
chromogenic oxygen acceptor such as O-dianisidine for the colorimetric determination of
glucose in body fluid was proposed from several investigators. In this report the glucose
level was determined in the non-and deproteinized serum and blood by means of
glucose oxidase method of the Sigma Company, also the recovery rate was examined by
addition of the standard glucose compatible to 50 to 100mg% with following results :
1. It was found that this method was available in the determination of blood sugar
without deprateinization in nonhemolytic serum.
2. When the known amounts of glucose solution were added to the serum and blood,
the recoveries were ranged from 90 to 110%.
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